Biology
187
General
Microbiology
Spring
2008
Course
Syllabus
Office: Rm 10D, Building 4
Phone:
515-964-6237
E-Mail: dlglassman@dmacc.edu
Web site:
http://www.dmacc.cc.ia.us/instructors/dlglassman/
Office Hours: Monday/Wednesday 9:00-10:00 &
12:15 - 1:15, Friday 9:00-10:00
Text:
Brock Biology of Microorganisms . 11th edition Madigan/Martinko/Parker
2006
Lab Manual: Laboratory
Experiments in Microbiology.
8th edition.
Johnson & Case.
2007
I. Lecture A tentative
lecture schedule is attached. You
are responsible for all material in the assigned chapters as well as the
material presented in class. If you
must miss a lecture it is your responsibility to obtain the notes from a
classmate. Attendance in lecture is
vital to your success.
II. Lab
You are required to attend each lab. A completed lab report for the previous
lab session is due at the beginning of the next lab session. Late lab reports can receive a maximum
of 50% of their value. You can
receive no points for a lab that you
did not attend!
III. Safety Laboratory
safety is of paramount importance.
It is essential that you follow proper procedures at all times. Some of the microorganisms you will be
handling are pathogenic! No food, drink, tobacco or gum can be
permitted in the lab. A lab coat or
smock must be worn during lab sessions.
Safety glasses or goggles must be worn when working with live
cultures. Read and commit to memory
the material in the handout “Microbiology Laboratory Rules and
Recommendations.”
IV. Web Site
http://www.dmacc.cc.ia.us/instructors/dlglassman/
The pertinent course information is also posted on my faculty web site. This site may be used throughout the semester to post information and assignments. You can also use this site as a link to other science oriented Web sites of interest.
V. Policy on Missed Exams,
Quizzes, and Labs:
NO makeup labs or quizzes will
be given. One missed exam may
be made up by adding the appropriate number of points missed to the value of the
final exam. Example: If one unit test is missed, the final
for that person will be worth 200 pts (value of final) + 100 pts (value of
missed exam) = 300 pts.
VI.
Competencies:
The attached competencies
are the minimum skills and knowledge that you must master to complete this
course. They do not reflect the degree of knowledge and understanding you must
demonstrate to earn a passing grade. If you have any questions concerning course
content or expectations do not hesitate to raise them.
VII. Grading There will be three (3) written
exams (100 points each) and a comprehensive final (200 points). Lab reports are worth 10 points each if
turned in on time and complete. Lab
exercise 8 requires identification of a morphologic unknown. This lab is worth 20 points. The lab report for the bacterial unknown
is worth 50 points. A comprehensive
practical will be given (100 points).
Quizzes and special assignments will also be given. You must pass both the lecture and
laboratory components of the course to pass the
course.
Final grades will be
assigned based on percentage of total points possible you
earn:
A
90-100
B
80-89
C
70-79
D
60-69
F
<60
Tentative point
totals
Lecture Exams
300
Final
200
Lab Reports
~180
Morphologic Unknown
20
Bacterial Unknown
50
Practical
100
Attendance, attitude, conduct and participation ~25
Special Assignments
~20
Biology 187
Very Tentative Lecture
Schedule
1
1/7
Chapters 1 & 2 Overview
of Microbiology
1/9
Chapter 3 – Macromolecules
2 1/14 Chapter 4 –Cell Structure and Function
1/16 Chapter 4 (cont.)
3
1/21
No Class – School
1/23 Chapter 5 – Nutrition and metabolism
4 1/28 Chapter 5 (cont.)
1/30 Chapter 6 – Microbial Growth
5
2/4
EXAM I
2/6 Chapter 7 – Microbial Molecular Biology
6 2/11 Chapter 7 (cont.)
2/13 Chapter 8 – Regulation of Gene Expression
7 2/18 Chapter 10 – Microbial Genetics
2/20 Chapter 10 (cont.)
8 2/25 Chapter 31 – Genetic Engineering and Biotechnology
2/27
Chapter 9 - Viruses
9
3/3
Chapter 9 (cont.)
3/5
EXAM II
10
3/10
Chapter 12 – Bacteria
3/12
Chapter 12
(cont.)
11
3/17-3/21
Spring Break ……No Classes
12
3/24
Chapter 12 (cont.)
3/26
Chapter 13 – Archaea
13 3/31 Chapter 14 - Eukaryotic microorganisms
4/2 Chapter 14 (cont.)
14 4/7 EXAM III
4/9 Chapters 18 & 19 Microbial Ecology
15 4/14 Chapter 18 & 19 (cont.)
4/16 Chapter 20 – Microbial Growth Control
16 4/21 Chapter 20 (cont.)
4/23 Chapter 22 – Immunology
17
FINAL EXAM
April 30th (Wednesday)
2:30-4:45
Biology 187 Laboratory Schedule
Spring 2008
DATE
LAB
TH 1-10
Ex. 2- Examination of
Living Organisms
TU 1-22
Ex. 6- Acid-Fast
Staining
Ex.
7- Structural Stains
(Endospore, Capsule, & Flagella)
TH 1-24
Ex. 8- Morphologic
Unknown
Ex. 26 - Effectiveness of Handscrubbing
Ex. 10- Transfer of Bacteria:
Aseptic Technique
Ex. 12- Special Media for Isolating
Bacteria
TU
2-12
Ex.
13- Carbohydrate
Catabolism
TH 2-14
Evaluate Ex. 13 and Ex. 14
TU 2-19
Ex. 15 Protein Catabolism, Part 1
Ex. 16 Protein Catabolism, Part 2
TH 2-21
Evaluate 15 & 16
TU 2-26
Ex. 17 Respiration
Ex. 18
Rapid Identification Methods
TH 2/28
Evaluate 17 and 18
TU 3-4
Identification of Unknown Microorganism - Handout
TH 3-6
Unknowns - continued
TU 3-11
Unknowns – conclusion
Ex. 19 Oxygen and the growth of Bacteria
TH 3-13
Evaluate Ex. 19
Ex. 43- Blood Group Determination:
Slide Agglutination
March 17 - March 21
SPRING BREAK!!!
Ex. 25- Chemical Methods of
Control: Disinfectants & Antiseptics
TH 3-27
Evaluate 24 and 25
TU 4-1
Virology Lab - Handout
TH 4-3
Evaluate Virology Lab
Ex.
36 Phototrophs
TU 4-8
Ex. 52- Microbes in Water: Multiple
Tube Analysis
Ex. 54 -
Microbes in Food: Contamination
TH 4-10
Evaluate 52 and 54
Ex. 57- Microbes in Soil:
Bioremediation
TU 4-15
Evaluate 57
Bacterial Transformation (pGLO) - Handout
TH 4-17
Evaluate Bacterial Transformation lab
DNA Restriction analysis - Handout
TU 4-22
Evaluate DNA Restriction analysis
Lab Cleanup/Review
TH 4-24
PRACTICAL
EXAM
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Disclaimer | ||
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“This
syllabus is representative of materials that will be covered in this
class; it is not a contract between the student and the institution. It is subject to change without
notice. Any potential
exceptions to stated policies and requirements will be addressed on an
individual basis, and only for reasons that meet specific
requirements. If you have any
problems related to this class, please feel free to discuss them with
me.” | ||
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Students
who wish additional information or assistance may contact the Executive Dean, Student Services,
Laurie Wolf, Building 1, 515-964-6437 or the EEO/AA Officer, Dr. Sandy Tryon,
Human Resources, Bldg. 1, 515-964-6301 or they may refer to Student
Services procedure 4645 located on the DMACC Intranet at http://my.dmacc.edu/procs.aspx. Go to Policies & Procedures and
choose Student Services Procedures. Employees and applicants who wish
additional information or assistance may contact the EEO/AA Officer, Dr. Sandy Tryon,
Human Resources, Bldg. 1, 515-964-6301 or refer to HR Procedures 3000,
3005, 3010, 3015, and 3020 at http://www.dmacc.edu/hr/hrpp.asp
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requests for accommodations, the Accommodation/Section 504/ADA
Coordinator, Sharon Bittner, can be contacted at 515-964-6857. Students with requests for
accommodations should refer to the Student Services procedure 4610 located
on the DMACC Intranet at http://my.dmacc.edu/default.aspx . Go to Policies & Procedures and
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Academic
Misconduct
Academic Integrity, based on
the values of honesty, trust, fairness, respect, and responsibility, is a
fundamental principle of scholarship at DMACC. DMACC’s Academic Misconduct
Policy prohibits: plagiarism (using another person’s writing or copying any work
without proper citation), falsification, unauthorized collaboration during a
test or on an assignment, or substitution for another student to take an exam,
course or test.
If you are to
benefit from this class and be properly evaluated for your contributions, it is
important for you to be familiar with and follow DMACC’s Academic Misconduct
policy. Students are encouraged to review DMACC’s Academic Misconduct Policy
on-line at http://www.dmacc.edu/handbook/polprocedures/es4670.pdf
or in the DMACC Student Handbook.
Work that
violates these values is incompatible with the goals of this class and will not be
tolerated. Students who are found responsible for a violation of the
Academic Misconduct Policy will receive a failing grade for the course. Students
have the right to appeal and may do so by following the procedures described in
the Academic Misconduct policy.
COURSE COMPETENCIES:
During this course, the student will be
expected to:
1. Identify the locations of safety
equipment in the laboratory.
2. Explain the safety procedures and health
rules of the microbiology laboratory.
3. Comply with safety and health rules of
the biology laboratory.
3.1 Observe appropriate safety procedures
for the microbiology laboratory.
3.2 Adhere to accepted health rules for the
microbiology laboratory.
4. Demonstrate an understanding of the use
of laboratory microscopes.
4.1 Identify the major parts of the
microscope.
4.2 Demonstrate proper technique in the use
of a microscope.
4.3 Demonstrate proper care of a
microscope.
5. Relate microbial morphology to function.
6. Classify disease causing microorganisms.
7. Differentiate between Gram-negative and
Gram-positive bacteria
8. Identify structures and functions of
endospores and plasmids.
9. Characterize microbial metabolism and
growth.
9.1 Summarize basic concepts of life
chemistry.
9.2 Relate cellular functions.
9.3 Discuss cellular respiration.
9.4 Differentiate among the metabolic
reactions of different
9.5 Identify nutrient needs of
disease-causing bacteria.
10. Relate microbial characteristics to
their disease-causing capabilities.
10.1 Relate Koch's postulates to modern
concepts of disease.
10.2 Explain terminology describing disease
processes.
10.3 Discuss infection.
11. Examine epidemiology.
12. Describe the normal flora of the human
body.
13. Differentiate among selected
disease-causing pathogens.
14. Identify methods of sterilization and
disinfection.
15. Identify anti-pathogenic
chemotherapeutic agents.
16. Explain the concept of host defense
mechanisms.
16.1 Identify the factors that are
important in disease resistance.
16.2 Differentiate between cell types
involved in immunity.
16.3 Specify the characteristics of
antibodies.
16.4 Differentiate between active and
passive immunity.
17. Utilize antiseptic techniques.
18. Demonstrate an understanding of
microbiology.
18.1 Demonstrate proper gram staining.
18.2 Prepare a bacteria culture.
19. Employ proper handwashing techniques.
20. Evaluate lab results.
21. Identify an unknown bacteria.
22. Discuss the importance of environmental and industrial
microbiology.